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Get cell boundaries in a noisy image?

POSTED BY: Ali Hashmi
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Detailed answer to this question was posted here:

Segment noisy microscope image into cell boundaries via valleys

POSTED BY: EDITORIAL BOARD

I encounter similar noise with astrophotography. I'm not sure about the origin of your image's noise, or if astro techniques will properly address the issue you are seeing but you might try a combination of:

1) Subtracting a dark frame (or an averaged set of dark frames). These are exposures of the same length as the 'light' frame, but with the camera blocked so that no light enters. It is probably irrelevant for your work, but your camera should be at the same temperature during the dark frames as the light frames. This will remove thermal noise associated with the CCD.

2) Subtract a 'bias' frame, which is like a dark frame but of extremely short duration. This frame removes noise intrinsic to the readout of the CCD's data.

3) Take a series of light frames and combine them using Mean or Median statistical function. This will average out the noise and improve the SNR.

POSTED BY: Tom Sherlock
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