Hello Matheus
1) I suggest NOT to use variables with subscripts, that may lead to problems
2) What is sol? Without sol your matrix rho is not defined
3) Here ( I arbritarily define a matrix rho ) is some code which seems to do what you want.
I introduced Abs in the eigenvalues because it turned out that complex values occured.
I hope this gives you hints how to proceed
rho[t_] := {
{1 + 1/t, 0, 0, t},
{0, t^2, 2 + t, 0},
{0, 2 + t, t^2, 0},
{3 t, 0, 0, 2 t}}
rho[x] // MatrixForm
ev[t_] := Eigenvalues[rho[t]]
ee[j_, t_] := Abs[ev[t][[j]]]
Plot[Sqrt[ee[1, t]] - Sqrt[ee[2, t]] - Sqrt[ee[3, t]] - Sqrt[ee[4, t]], {t, 1, 100}]
Plot[Max[-20, Sqrt[ee[1, t]] - Sqrt[ee[2, t]] - Sqrt[ee[3, t]] - Sqrt[ee[4, t]]], {t, 1, 100}]
And in abbreviated form:
Plot[Max[-20, Re@({1, -1, -1, -1}.Sqrt[Abs[ev[t]]])], {t, 1, 100}]