Also, if a list of results is what you really want, here are two more methods:
In[1]:= x = Range[1, 10];
In[2]:= Table[x[[n]] - x[[n - 4]], {n, 5, Length[x]}]
Out[2]= {4, 4, 4, 4, 4, 4}
In[3]:= ListCorrelate[{-1, 0, 0, 0, 1}, x]
Out[3]= {4, 4, 4, 4, 4, 4}