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Extract nuclei from images with dark cytoplasm?

Posted 3 months ago
2 Replies
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Hello and thank you in advance for your help. Please, see, for instance, the following two images.

Cell 1 Cell2

Any ideas for a correct isolation of the nuclei? Unfortunately, these images are from our archive and they cannot be recaptured. The sample also cannot be re-prepared. This is a “tricky” challenge. Nothing seems to work for a correct isolation.

2 Replies

Hi Sotirios,

here comes a simple first guess:

web = {"\
imgs0 = Import /@ web;
thrh = FindThreshold /@ imgs0;
nucs = ColorNegate /@ MapThread[MorphologicalBinarize, {imgs0, thrh}];
MapThread[HighlightImage, {imgs0, nucs}]

This gives:

enter image description here

Does that help?

Regards -- Henrik

Posted 3 months ago

Dear Henrik,

Thank you very much for your very valuable help!!!

I will inform you about the results.

Thank you once again!

Best regards, Sotirios

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