Thanks Itai and John. I was trying every combination of using Tab except the correct one!
The issue with \[NegativeVeryThinSpace], as well as corresponding issues with the other negative spacing characters, has been fixed for the 10.0.1 release. Which I can't put a precise date on, yet, but you'll probably see it sometime next month.
OK, thanks David, I missed that. Well, they're not exactly in an obvious location.
Subtitle and Subsubtitle are still in the Format>Style menu but they are now located below the Output style listing:
Thanks Vitaliy, but it's seems to be another Windows problem. In my Formatting Toolbar neither Subtitle nor Subsubtitle appear in the menu. Nor do they appear on any other Style menus.
And one really shouldn't have to do this by programming.
You can do this programmatically:
CellPrint[Cell["Test", "Subtitle"]]
But via interface for standar workflow - this is one of the ways: